Plasmid Preparation:Article Title: The Rnf Complex is Vital for Metabolic Adaptation and Virulence in the Oral Pathogen Fusobacterium nucleatum
Article Snippet: Appendix Table B-1: Response Regulators in F. nucleatum ATCC 23726 Gene ID Homolog Type Associated Processa Reference C4N14_RS05840 ArlR Adhesion, autolysis, proteolysis (1, 2) C4N14_RS09320 CarR Lysine metabolism, coaggregation (3) C4N14_RS07475 CheY Chemotaxis (4) C4N14_RS02760 EutV Ethanolamine utilization (5) C4N14_RS04330 ModR Oxidative stress response (6) C4N14_RS02660 S1 RNA binding Transcription This study C4N14_RS04495 YpdB Autolysis (7) aBased on the available information of homologous response regulators. .. Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Wild-type (WT) strain (8) F. nucleatum CW1 ∆galK; an isogenic derivative of 23726 (8) F. nucleatum fap2::Tn5 Derivative of 23726 with Tn5 insertion mapped to fap2 at position 2054 (11361) (3) F. nucleatum ∆rnfC Isogenic derivative of CW1 lacking rnfC (9) F. nucleatum ∆fadA Isogenic derivative of CW1 lacking fadA This study F. nucleatum ∆carR Isogenic derivative of CW1 lacking carR (3) F. nucleatum ∆modR Isogenic derivative of CW1 lacking modR (6) F. nucleatum ∆arlR Isogenic derivative of CW1 lacking arlR This study F. nucleatum ∆cheY Isogenic derivative of CW1 lacking cheY This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study F. nucleatum ∆s1 Isogenic derivative of CW1 lacking s1 This study F. nucleatum ∆ypdB Isogenic derivative of CW1 lacking ypdB This study Plasmid pCWU6 Derivative of pHS30 (8) pMCSG7 ligation-independent cloning vector (10) pZP4C CRISPR-based vector (11) pCM-GalK C. perfringens vector expressing galK (8) pMCSG7-FadA pMCSG7 expressing FadA This study pMCSG7-CarR pMCSG7 expressing CarR This study pMCSG7-Fap2 pMCSG7 expressing Fap2 This study pRnfC pCWU6 expressing RnfC under the control of the rpsJ promoter (9) pZP4C-lepB Derivative of pZP4C for lepB depletion This study pGalK-∆fadA pCM-galK derivative; rnfC deletion vector This study pGalK-∆arlR pCM-galK derivative; arlR deletion vector This study pGalK-∆cheY pCM-galK derivative; cheY deletion vector This study pGalK-∆eutV pCM-galK derivative; eutV deletion vector This study pGalK-∆s1 pCM-galK derivative; s1 deletion vector This study pGalK-∆ypdB pCM-galK derivative; ypdB deletion vector This study 87 ..
Ligation:Article Title: The Rnf Complex is Vital for Metabolic Adaptation and Virulence in the Oral Pathogen Fusobacterium nucleatum
Article Snippet: Appendix Table B-1: Response Regulators in F. nucleatum ATCC 23726 Gene ID Homolog Type Associated Processa Reference C4N14_RS05840 ArlR Adhesion, autolysis, proteolysis (1, 2) C4N14_RS09320 CarR Lysine metabolism, coaggregation (3) C4N14_RS07475 CheY Chemotaxis (4) C4N14_RS02760 EutV Ethanolamine utilization (5) C4N14_RS04330 ModR Oxidative stress response (6) C4N14_RS02660 S1 RNA binding Transcription This study C4N14_RS04495 YpdB Autolysis (7) aBased on the available information of homologous response regulators. .. Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Wild-type (WT) strain (8) F. nucleatum CW1 ∆galK; an isogenic derivative of 23726 (8) F. nucleatum fap2::Tn5 Derivative of 23726 with Tn5 insertion mapped to fap2 at position 2054 (11361) (3) F. nucleatum ∆rnfC Isogenic derivative of CW1 lacking rnfC (9) F. nucleatum ∆fadA Isogenic derivative of CW1 lacking fadA This study F. nucleatum ∆carR Isogenic derivative of CW1 lacking carR (3) F. nucleatum ∆modR Isogenic derivative of CW1 lacking modR (6) F. nucleatum ∆arlR Isogenic derivative of CW1 lacking arlR This study F. nucleatum ∆cheY Isogenic derivative of CW1 lacking cheY This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study F. nucleatum ∆s1 Isogenic derivative of CW1 lacking s1 This study F. nucleatum ∆ypdB Isogenic derivative of CW1 lacking ypdB This study Plasmid pCWU6 Derivative of pHS30 (8) pMCSG7 ligation-independent cloning vector (10) pZP4C CRISPR-based vector (11) pCM-GalK C. perfringens vector expressing galK (8) pMCSG7-FadA pMCSG7 expressing FadA This study pMCSG7-CarR pMCSG7 expressing CarR This study pMCSG7-Fap2 pMCSG7 expressing Fap2 This study pRnfC pCWU6 expressing RnfC under the control of the rpsJ promoter (9) pZP4C-lepB Derivative of pZP4C for lepB depletion This study pGalK-∆fadA pCM-galK derivative; rnfC deletion vector This study pGalK-∆arlR pCM-galK derivative; arlR deletion vector This study pGalK-∆cheY pCM-galK derivative; cheY deletion vector This study pGalK-∆eutV pCM-galK derivative; eutV deletion vector This study pGalK-∆s1 pCM-galK derivative; s1 deletion vector This study pGalK-∆ypdB pCM-galK derivative; ypdB deletion vector This study 87 ..
Cloning:Article Title: The Rnf Complex is Vital for Metabolic Adaptation and Virulence in the Oral Pathogen Fusobacterium nucleatum
Article Snippet: Appendix Table B-1: Response Regulators in F. nucleatum ATCC 23726 Gene ID Homolog Type Associated Processa Reference C4N14_RS05840 ArlR Adhesion, autolysis, proteolysis (1, 2) C4N14_RS09320 CarR Lysine metabolism, coaggregation (3) C4N14_RS07475 CheY Chemotaxis (4) C4N14_RS02760 EutV Ethanolamine utilization (5) C4N14_RS04330 ModR Oxidative stress response (6) C4N14_RS02660 S1 RNA binding Transcription This study C4N14_RS04495 YpdB Autolysis (7) aBased on the available information of homologous response regulators. .. Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Wild-type (WT) strain (8) F. nucleatum CW1 ∆galK; an isogenic derivative of 23726 (8) F. nucleatum fap2::Tn5 Derivative of 23726 with Tn5 insertion mapped to fap2 at position 2054 (11361) (3) F. nucleatum ∆rnfC Isogenic derivative of CW1 lacking rnfC (9) F. nucleatum ∆fadA Isogenic derivative of CW1 lacking fadA This study F. nucleatum ∆carR Isogenic derivative of CW1 lacking carR (3) F. nucleatum ∆modR Isogenic derivative of CW1 lacking modR (6) F. nucleatum ∆arlR Isogenic derivative of CW1 lacking arlR This study F. nucleatum ∆cheY Isogenic derivative of CW1 lacking cheY This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study F. nucleatum ∆s1 Isogenic derivative of CW1 lacking s1 This study F. nucleatum ∆ypdB Isogenic derivative of CW1 lacking ypdB This study Plasmid pCWU6 Derivative of pHS30 (8) pMCSG7 ligation-independent cloning vector (10) pZP4C CRISPR-based vector (11) pCM-GalK C. perfringens vector expressing galK (8) pMCSG7-FadA pMCSG7 expressing FadA This study pMCSG7-CarR pMCSG7 expressing CarR This study pMCSG7-Fap2 pMCSG7 expressing Fap2 This study pRnfC pCWU6 expressing RnfC under the control of the rpsJ promoter (9) pZP4C-lepB Derivative of pZP4C for lepB depletion This study pGalK-∆fadA pCM-galK derivative; rnfC deletion vector This study pGalK-∆arlR pCM-galK derivative; arlR deletion vector This study pGalK-∆cheY pCM-galK derivative; cheY deletion vector This study pGalK-∆eutV pCM-galK derivative; eutV deletion vector This study pGalK-∆s1 pCM-galK derivative; s1 deletion vector This study pGalK-∆ypdB pCM-galK derivative; ypdB deletion vector This study 87 ..
CRISPR:Article Title: The Rnf Complex is Vital for Metabolic Adaptation and Virulence in the Oral Pathogen Fusobacterium nucleatum
Article Snippet: Appendix Table B-1: Response Regulators in F. nucleatum ATCC 23726 Gene ID Homolog Type Associated Processa Reference C4N14_RS05840 ArlR Adhesion, autolysis, proteolysis (1, 2) C4N14_RS09320 CarR Lysine metabolism, coaggregation (3) C4N14_RS07475 CheY Chemotaxis (4) C4N14_RS02760 EutV Ethanolamine utilization (5) C4N14_RS04330 ModR Oxidative stress response (6) C4N14_RS02660 S1 RNA binding Transcription This study C4N14_RS04495 YpdB Autolysis (7) aBased on the available information of homologous response regulators. .. Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Wild-type (WT) strain (8) F. nucleatum CW1 ∆galK; an isogenic derivative of 23726 (8) F. nucleatum fap2::Tn5 Derivative of 23726 with Tn5 insertion mapped to fap2 at position 2054 (11361) (3) F. nucleatum ∆rnfC Isogenic derivative of CW1 lacking rnfC (9) F. nucleatum ∆fadA Isogenic derivative of CW1 lacking fadA This study F. nucleatum ∆carR Isogenic derivative of CW1 lacking carR (3) F. nucleatum ∆modR Isogenic derivative of CW1 lacking modR (6) F. nucleatum ∆arlR Isogenic derivative of CW1 lacking arlR This study F. nucleatum ∆cheY Isogenic derivative of CW1 lacking cheY This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study F. nucleatum ∆s1 Isogenic derivative of CW1 lacking s1 This study F. nucleatum ∆ypdB Isogenic derivative of CW1 lacking ypdB This study Plasmid pCWU6 Derivative of pHS30 (8) pMCSG7 ligation-independent cloning vector (10) pZP4C CRISPR-based vector (11) pCM-GalK C. perfringens vector expressing galK (8) pMCSG7-FadA pMCSG7 expressing FadA This study pMCSG7-CarR pMCSG7 expressing CarR This study pMCSG7-Fap2 pMCSG7 expressing Fap2 This study pRnfC pCWU6 expressing RnfC under the control of the rpsJ promoter (9) pZP4C-lepB Derivative of pZP4C for lepB depletion This study pGalK-∆fadA pCM-galK derivative; rnfC deletion vector This study pGalK-∆arlR pCM-galK derivative; arlR deletion vector This study pGalK-∆cheY pCM-galK derivative; cheY deletion vector This study pGalK-∆eutV pCM-galK derivative; eutV deletion vector This study pGalK-∆s1 pCM-galK derivative; s1 deletion vector This study pGalK-∆ypdB pCM-galK derivative; ypdB deletion vector This study 87 ..
Expressing:Article Title: The Rnf Complex is Vital for Metabolic Adaptation and Virulence in the Oral Pathogen Fusobacterium nucleatum
Article Snippet: Appendix Table B-1: Response Regulators in F. nucleatum ATCC 23726 Gene ID Homolog Type Associated Processa Reference C4N14_RS05840 ArlR Adhesion, autolysis, proteolysis (1, 2) C4N14_RS09320 CarR Lysine metabolism, coaggregation (3) C4N14_RS07475 CheY Chemotaxis (4) C4N14_RS02760 EutV Ethanolamine utilization (5) C4N14_RS04330 ModR Oxidative stress response (6) C4N14_RS02660 S1 RNA binding Transcription This study C4N14_RS04495 YpdB Autolysis (7) aBased on the available information of homologous response regulators. .. Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Wild-type (WT) strain (8) F. nucleatum CW1 ∆galK; an isogenic derivative of 23726 (8) F. nucleatum fap2::Tn5 Derivative of 23726 with Tn5 insertion mapped to fap2 at position 2054 (11361) (3) F. nucleatum ∆rnfC Isogenic derivative of CW1 lacking rnfC (9) F. nucleatum ∆fadA Isogenic derivative of CW1 lacking fadA This study F. nucleatum ∆carR Isogenic derivative of CW1 lacking carR (3) F. nucleatum ∆modR Isogenic derivative of CW1 lacking modR (6) F. nucleatum ∆arlR Isogenic derivative of CW1 lacking arlR This study F. nucleatum ∆cheY Isogenic derivative of CW1 lacking cheY This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study F. nucleatum ∆s1 Isogenic derivative of CW1 lacking s1 This study F. nucleatum ∆ypdB Isogenic derivative of CW1 lacking ypdB This study Plasmid pCWU6 Derivative of pHS30 (8) pMCSG7 ligation-independent cloning vector (10) pZP4C CRISPR-based vector (11) pCM-GalK C. perfringens vector expressing galK (8) pMCSG7-FadA pMCSG7 expressing FadA This study pMCSG7-CarR pMCSG7 expressing CarR This study pMCSG7-Fap2 pMCSG7 expressing Fap2 This study pRnfC pCWU6 expressing RnfC under the control of the rpsJ promoter (9) pZP4C-lepB Derivative of pZP4C for lepB depletion This study pGalK-∆fadA pCM-galK derivative; rnfC deletion vector This study pGalK-∆arlR pCM-galK derivative; arlR deletion vector This study pGalK-∆cheY pCM-galK derivative; cheY deletion vector This study pGalK-∆eutV pCM-galK derivative; eutV deletion vector This study pGalK-∆s1 pCM-galK derivative; s1 deletion vector This study pGalK-∆ypdB pCM-galK derivative; ypdB deletion vector This study 87 ..
Control:Article Title: The Rnf Complex is Vital for Metabolic Adaptation and Virulence in the Oral Pathogen Fusobacterium nucleatum
Article Snippet: Appendix Table B-1: Response Regulators in F. nucleatum ATCC 23726 Gene ID Homolog Type Associated Processa Reference C4N14_RS05840 ArlR Adhesion, autolysis, proteolysis (1, 2) C4N14_RS09320 CarR Lysine metabolism, coaggregation (3) C4N14_RS07475 CheY Chemotaxis (4) C4N14_RS02760 EutV Ethanolamine utilization (5) C4N14_RS04330 ModR Oxidative stress response (6) C4N14_RS02660 S1 RNA binding Transcription This study C4N14_RS04495 YpdB Autolysis (7) aBased on the available information of homologous response regulators. .. Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Wild-type (WT) strain (8) F. nucleatum CW1 ∆galK; an isogenic derivative of 23726 (8) F. nucleatum fap2::Tn5 Derivative of 23726 with Tn5 insertion mapped to fap2 at position 2054 (11361) (3) F. nucleatum ∆rnfC Isogenic derivative of CW1 lacking rnfC (9) F. nucleatum ∆fadA Isogenic derivative of CW1 lacking fadA This study F. nucleatum ∆carR Isogenic derivative of CW1 lacking carR (3) F. nucleatum ∆modR Isogenic derivative of CW1 lacking modR (6) F. nucleatum ∆arlR Isogenic derivative of CW1 lacking arlR This study F. nucleatum ∆cheY Isogenic derivative of CW1 lacking cheY This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study F. nucleatum ∆s1 Isogenic derivative of CW1 lacking s1 This study F. nucleatum ∆ypdB Isogenic derivative of CW1 lacking ypdB This study Plasmid pCWU6 Derivative of pHS30 (8) pMCSG7 ligation-independent cloning vector (10) pZP4C CRISPR-based vector (11) pCM-GalK C. perfringens vector expressing galK (8) pMCSG7-FadA pMCSG7 expressing FadA This study pMCSG7-CarR pMCSG7 expressing CarR This study pMCSG7-Fap2 pMCSG7 expressing Fap2 This study pRnfC pCWU6 expressing RnfC under the control of the rpsJ promoter (9) pZP4C-lepB Derivative of pZP4C for lepB depletion This study pGalK-∆fadA pCM-galK derivative; rnfC deletion vector This study pGalK-∆arlR pCM-galK derivative; arlR deletion vector This study pGalK-∆cheY pCM-galK derivative; cheY deletion vector This study pGalK-∆eutV pCM-galK derivative; eutV deletion vector This study pGalK-∆s1 pCM-galK derivative; s1 deletion vector This study pGalK-∆ypdB pCM-galK derivative; ypdB deletion vector This study 87 ..
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